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Radnoti、158700-1、Blood Vessel Perfusion System隔離血管灌注系統
Radnoti、158700-1、Blood Vessel Perfusion System隔離血管灌注系統
  • Radnoti、158700-1、Blood Vessel Perfusion System隔離血管灌注系統

Radnoti、158700-1、Blood Vessel Perfusion System隔離血管灌注系統

產品報價:詢價

更新時間:2019/10/11 11:16:58

地:美國

牌:Radnoti

號:158700-1

廠商性質: 生產型,貿易型,服務型,

公司名稱: 北京容圣科技有限公司

產品關鍵詞: Blood Vessel Perfusion   隔離血管灌注系統   Radnoti  

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程園 : (18611717737) (010-57129142)

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Radnoti隔離血管灌注系統設計用于允許研究人員在溫度受控的環境中測量血管段的收縮反應。血管或其他管狀肌肉結構(例如小腸段)安裝在穿過浴帽的一對玻璃套管上。每套有一個筆直的套管和一個J形套管;血管連接到J形的末端并連接到筆直的套管,從而形成一個環,血管可以通過該環進行灌注。通過以設定的速率灌注血管,可以通過在線壓力傳感器記錄的壓力變化來檢測血管直徑的變化。安裝套管的位置可通過使用浴帽中內置的螺絲鎖定位套管來適應容器的長度,將血管末端插入每個插管的末端,并將血管綁到位。蓋子還具有位于血管兩側的鉑場電極,可對肌肉進行電刺激。然后將帶有容器的蓋子插入溫度控制槽中。

浴槽具有允許連續或作為離散洗滌液交換浴液的入口。因此,如果需要,可以向容器內部和外部灌注相似或不同的流體(即使在不同的溫度下),并且可以連續收集流體。流體也可以獨立充氣,使研究人員可以創建不同的內部和外部有氧環境

Radnoti Glass Technology開發了一種特殊的灌注系統,以滿足對內皮功能,內源性神經遞質和血管活性物質的代謝和釋放迅速發展的血管研究感興趣的科學家的需求。

該系統包括一個Radnoti 25毫升器官浴,該浴池的水套中裝有灌注液預熱盤管。溶液通過玻璃魯爾接頭流出,然后通過連接管進入入口套管。在預熱線圈的入口和出口使用Luer配件可使該系統與另一個灌注系統相連,從而形成一個級聯系統(請參見方框)。

這種配置還允許放置壓力傳感器,并允許在灌注介質到達血管之前添加或移除灌注介質。灌注系統還具有帶可調節針閥的可更換曝氣器,底部排水孔和溢流出口。灌注系統蓋由高密度聚丙烯制成,并帶有O形圈密封以確保緊密貼合。蓋子上有五個孔,用于穿過一組刺激電極以及一個流入和流出套管(在其間安裝有血管)和一個用于將樣本添加到浴槽內部的管。插入蓋中的所有物品都可以使用尼龍緊定螺釘定位。

為了使用該灌注系統,將選擇的血管解剖并清洗。然后通過縫合將血管固定到流入和流出套管上。流入套管是直的,而流出套管的一端是鉤狀的,以利于正確對準血管。插管的兩端均略微張開,并具有精細的研磨表面,以確保與血管的牢固配合。

標準插管的外徑為1.0、1.5、2.0、2.5和3.0mm,可容納各種制劑,例如大鼠尾動脈,門靜脈,肺動脈和腸系膜或孤立的大血管。可根據要求提供其他尺寸。該設計采用了開放式灌注系統,用于研究血管收縮劑和血管擴張劑,神經刺激以及各種藥物在體外對整個血管床的作用。

刺激電極位于組織中,以促進神經遞質的釋放。當安裝組織時,將電極向上滑動并用固定螺釘固定,從而可以清楚地進入套管。一旦組織被固定,電極就被重新定位。可以通過使用底部排水孔或溢流出口以及通過移液器,注射器或外部容器添加的溶液來快速更換浴液。

用一組插管中的一個插管該容器部分,并將其置于可充氣的水套浴中。在恒壓模式下,將儲液罐(或氣泡收集器)放在給定靜水壓頭的熔池上方,并通過容器的收縮或膨脹來改變流量。流量測量是使用降落計數器,流量計或其他設備進行的。在恒定流量模式下,蠕動泵或注射泵以預定速率輸送流體。在線壓力傳感器通過壓力變化測量直徑變化。

在恒定流量系統中,可以通過注射泵將藥物注入系統中。在兩種模式下,均可獨立于通過血管灌注的介質更換血管外部的介質。也可以使用攝像機直接測量容器的直徑。請注意,在某些情況下,可以使用水族館泵為非碳酸氫鹽緩沖系統中的氧氣需求量低的組織充氣,從而消除了氣罐和調節器的問題和費用。

四通道系統中包含的儀器包括:4個壓力傳感器,蠕動泵,儲液器,氣泡收集器,循環浴以及所有相關的管道和連接器。單通道系統不包括泵或循環浴。可用選項:用于組織刺激的留置電極,組織刺激器,流量計或其他流體測量設備,照相機,氣泵以及自動化的數據采集。

血管灌注系統有兩種尺寸:
158700-1單通道血管灌注系統
158700-4四通道血管灌注系統


The Radnoti isolated blood vessel perfusion system is designed to permit the researcher to measure the contractile responses of blood vessel segments in a temperature controlled environment. Blood vessels, or other tubular muscular structures such as small sections of intestine, are mounted on a pair of glass cannula that pass through the cap of the bath. There is one straight cannula and one J-shaped cannula per set; the vessel is attached over the end of the J-shape and connected to the straight cannula, thus forming a loop through which the vessel can be perfused. By perfusing the vessel at a set rate, changes in vessel diameter can be detected by pressure changes registered by in-line pressure transducers. The mounting cannula are set in position to accommodate the vessel’s length by positioning the cannula using the screw locks built into the cap of the bath, inserting the ends of the vessel over the end of each cannula and tying the vessel onto position. The cap also has platinum field electrodes positioned on either side of the vessel, permitting electrical stimulation of the muscle. The cap with attached vessel is then inserted into the temperature controlled bath.

The bath has access ports that permit exchange of the bath fluid, either continuously or as discrete washes. Therefore, the vessel can be perfused internally and externally with similar or different fluids (even at different temperatures) and the fluids can be collected on a continual basis, if desired. The fluids can also be gassed independently, allowing the researcher to create different internal and external aerobic environments

Radnoti Glass Technology has developed a special perfusion system to meet the needs of scientists interested in blood vessel research in the rapidly advancing areas of endothelial function, metabolism and release of endogenous neurotransmitters and vasoactive substances.

This system includes a Radnoti 25ml organ bath, which has a perfusate pre-warming coil built into the bath’s water-jacket. The solution exits through a glass Luer fitting before passing into the entry cannula through connecting tubing. The use of Luer fittings on the entry and exit of the pre-warming coil permit the system to be coupled to another perfusion system to form a cascade system (see Box).

This configuration also allows placement of a pressure transducer and permits addition or removal of perfusion media before it reaches the blood vessel. The perfusion system also has a replaceable aerator with adjustable needle valve, a bottom drain and overflow outlet. The perfusion system cap is made of high-density polypropylene with an O-ring seal to insure a snug fit. The cap has five holes drilled through it for passage of a set of stimulating electrodes and an inflow and outflow cannula, between which the blood vessel is mounted, and a tube for sample addition to the bath interior. All of the items inserted into the cap can be positioned through the use of nylon set screws.

To use this perfusion system, the blood vessel of choice is dissected out and cleaned. The vessel is then secured to the inflow and the outflow cannula by means of a suture. The inflow cannula is straight while the outflow has a hooked end to facilitate proper vessel alignment. The ends of the cannula are both slightly flared with a fine ground finish to insure a secure fit to the blood vessel.

Standard cannula O.D.’s are 1.0, 1.5, 2.0, 2.5 and 3.0mm which will accommodate various preparations such as rat tail artery, portal vein, pulmonary aorta, and the mesentery or isolated large vessels. Additional sizes are available upon request. This design incorporates an open-end perfusion system for the study of vasoconstrictors and vasodilators, nerve stimulation, and the effect of various drugs on an entire vascular bed in-vitro.

Stimulating electrodes are positioned in the tissue to promote the release of neurotransmitters. When mounting the tissue, the electrodes are slid upward and secured with set screws, providing clear access to the cannula. Once the tissue is secured, the electrodes are repositioned. The bath media can be rapidly exchanged through the use of the bottom drain or the overflow outlet and solutions added via pipette, syringe or external reservoir.

The vessel section is cannulated with one of a set of cannula and placed in a water-jacketed bath that can be aerated. In the constant pressure mode, a reservoir (or bubble trap) is placed above the bath at a given hydrostatic head and flow is changed by the contraction or dilation of the vessel. Flow measurements are made using drop counters, flow meters or other devices. In the constant flow mode, a peristaltic or syringe pump delivers fluid at a predetermined rate. In-line pressure transducers measure changes in diameter via changes in pressure.

In the constant flow system, drugs may be infused into the system via syringe pumps. In both modes, the media outside the vessels can be changed independently of the media perfused through the vessels. It is also possible to directly measure the diameter of the vessel using a camera. Note that in certain cases an aquarium pump can be used to aerate tissues with low oxygen requirements in non-bicarbonate buffer systems, thereby eliminating the problems and expense of gas tanks and regulators.

Instrumentation included in the Four Channel system includes: 4 pressure transducers, a peristaltic pump, reservoirs, bubble traps, recirculating bath, and all associated tubing and connectors. The Single Channel system does not include pumps, or recirculating bath. Available options: Indwelling electrodes for tissue stimulation, a tissue stimulator, flow meters, or other fluid measuring devices, camera, air pump, and data acquisition with automation.

The Blood Vessel Perfusion System is available in two sizes:
158700-1 Single Channel Blood Vessel Perfusion System
158700-4 Four Channel Blood Vessel Perfusion System



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